Snap cell - Cell. 2006 Dec 15;127(6):1286-7. doi: 10.1016/j.cell.2006.12.005. Authors Matthew S Hayden 1 , A Phillip West, Sankar Ghosh. Affiliation 1 Departments of Immunobiology and Molecular Biophysics and Biochemistry, Yale University School of Medicine, NewHaven, CT 06519, USA. PMID: 17174900 DOI ...

 
Sep 20, 2022 ... 1 Answer 1 ... In the end, I didn't completely solve this problem, but found a work-around. Rather than using the cell size and snap raster .... River mark community credit union

Reporting practices for publishing results with human PSCs and tissue stem cells. This checklist is intended to help scientists, reviewers, and editors prepare and assess manuscripts for inclusion of critical details relevant to work with pluripotent stem cells (PSCs) and tissue stem cells (TSCs) with the goal of increasing the rigor and ...Blocking agents are non-fluorescent compounds that block the reactivity of the SNAP- or CLIP-tag intracellularly (SNAP-Cell® Block, NEB #S9106; and CLIP-Cell™ Block, NEB #S9220) or on the surface of live cells (SNAP-Surface® Block, NEB #S9143; and CLIP-Cell Block).They can be used to generate inactive controls in live cell labeling experiments performed with SNAP- or …Atlas DX. Atlas DX is a cloud-based platform that powers Affinitiv's sales, service, and retention marketing solutions for the automotive industry. This single, fully integrated platform is built on predictive intelligence to promote initial purchase, repurchase, and long-term loyalty, accelerating performance for OEMs and dealers.Cell Division: Stages of Meiosis in a Snap! Unlock the full A-level Biology course at http://bit.ly/2OFjitm created by Adam Tildesley, Biology expert at Snap...SNAP Cell. The SNAP-tag ® system is based on a DNA repair enzyme, O 6 -alkylguanine-DNA alkyltransferase (AGT). It allows for multiple substrate options to enable color changes. It is highly temperature and fixation stable and can be used in vitro or in vivo . The substrate consists of two parts; the benzylguanine group and the functional ...Jul 27, 2020 · SnapCell is a video lead generation platform that enables dealers to create walkaround videos, technician videos, virtual test drives, and even talk to customers through live two-way video chat directly from a smartphone. Our entire suite of video and intuitive editing tools is entirely accessible from the app. Stay connected for free. with Lifeline Service from Assurance Wireless. FREE Smartphone*. FREE Unlimited Text. FREE 1,000 Talk Minutes. FREE 4.5GB Data. All on the T-Mobile Network. Data and Talk minutes may vary based on state. Enhanced offers apply in CA, TX, UT, WI – click "Apply Now" to see state specific offers.Snapcell. Tampa, Florida, United States. website. Snapcell is a SaaS automotive video marketing platform that helps car dealerships build trust and improve customer engagement through personalized video solutions. Snapcell was founded in 2016 and is based in Tampa, Florida. Deal Type. Try For Free 7-Day Free Trial. Use of SNAP-Cell Block with SNAP-Cell Substrates (E9100) Use with CLIP-tag substrates (S9220) View the video "Fluorescent Labeling of COS-7 Expressing SNAP-tag Fusion Proteins for Live Cell Imaging" in the Journal of Visualized Experiments (JoVE) Instructions for Cellular Labeling (E9200) Labeling Proteins in vitro (E9200) A dealer and franchise locator is available for Snap-on tools through the official Snap-on website at snapon.com; however, the user will need to complete all of the required fields...UMX cell phones cases are available at a variety of online and offline retailers, such as Amazon and Wal-Mart. Amazon offers a large selection of cases for the UMX cell phones, inc...Choose a Payment Plan: FULL PRICE TODAY $49. Trade-in to TradeUp! Get up to $700 on eligible trade-ins! Call (888) 583-0092 or click to learn more. Add to Cart. Explore available cell phones and plans from Consumer Cellular. Stay in touch with affordable, no-risk cell plans and phones from Consumer Cellular.Blocking agents are non-fluorescent compounds that block the reactivity of the SNAP- or CLIP-tag intracellularly (SNAP-Cell® Block, NEB #S9106; and CLIP-Cell™ Block, NEB #S9220) or on the surface of live cells (SNAP-Surface® Block, NEB #S9143; and CLIP-Cell Block).They can be used to generate inactive controls in live cell labeling experiments performed with SNAP- or …Here at SnapCell, as we put together a guide to creating the perfect walk around video, we realized this is an essential part of video’s growing demand. After all, this need for better instructions is just one of the reasons ‘how to’, unboxing, and other video content based on walk arounds are so popular. But they also provide dealers ...SNAP-Cell ® TMR-Star is a red fluorescent substrate that can be used to label SNAP-tag ® fusion proteins inside living cells, on cell surfaces, or in vitro. It is a cell-permeable substrate (CP-6-TMR) is based on the single isomer 6 …Mar 4, 2010 ... We first tested the site-specific labeling of various SNAP fusion proteins with control STG1 and STG2 dyes in live HEK293T cells. To our ...15 Feb, 2021, 08:33 ET. TAMPA, Fla., Feb. 15, 2021 /PRNewswire/ -- Lead generation and video tool, SnapCell, has been selected by GM Canada to be a certified partner for visual solutions as part ...When compared to FlickFusion and other car dealer software providers, Snapcell Live stands out thanks to the following features, High-quality video, Multi-person live streams, Screen sharing, No customer downloadsThe engineered homozygous clone expressing SOD1-SNAP fusion proteins has normal stem cell morphology and karyotype, expresses pluripotency markers, and can be ...Site-specific labeling of cellular proteins with chemical probes is a powerful tool for live cell imaging of biological processes. One popular system, known as the SNAP-tag, is based on an engineered variant of the 20-kDa DNA repair protein O 6-alkylguanine-DNA-alkyltransferase (AGT) that covalently reacts with O 6-benzylguanine (BG) and can be …SnapCell delivers a speedy solution, giving automotive businesses the chance to effectively and easily reach a wider audience via video content. While traditional marketing techniques like leafleting and posters remain effective, videos allow dealerships to promote current offers, deals and up-and-coming events to both new and loyal customers.Snap-on tools are known for their durability and reliability, but even the best tools can sometimes break or malfunction. That’s why it’s important to take advantage of your Snap-o...... SNAP, is a cytoplasm and cell membrane protein that belongs to the SNAP-25 family. SNAP25 / SUP contains 2 t-SNARE coiled-coil homology domains. SNAP25 ...What was this rocket's fatal flaw? A steel strut holding a helium bottle inside a SpaceX Falcon 9 rocket snapped during flight, setting off a chain reaction that destroyed the rock...The SNAP-tag is a small protein based on mammalian O 6 -alkylguanine-DNA alkyltransferase (AGT). SNAP-tag substrates are derivatives of benzylpurines and … Snapcell Corporate. 400 N Tampa St, Floor 15 Tampa, Florida 33602. email: [email protected] phone: (813) 303-0181 SNAP-Cell ® TMR-Star is a red fluorescent substrate that can be used to label SNAP-tag ® fusion proteins inside living cells, on cell surfaces, or in vitro. It is a cell-permeable substrate (CP-6-TMR) is based on the single isomer 6-carboxytetramethylrhodamine. It has an excitation maximum at 554 nm and an emission maximum at 580 nm.SnapCell is a simple & reliable automotive video tool that increases: - Dealership Revenue. - Showroom Visits. - Service Upsell. - CSI Scores. Download the app today and begin to …This checklist is intended to help scientists, reviewers, and editors prepare and assess manuscripts for inclusion of critical details relevant to work with pluripotent stem cells …Product Listing Application Overview. The SNAP-tag ® system is based on a DNA repair enzyme, O 6 -alkylguanine-DNA alkyltransferase (AGT). It allows for multiple substrate …The engineered homozygous clone expressing SOD1-SNAP fusion proteins has normal stem cell morphology and karyotype, expresses pluripotency markers, and can be ...SNAP-23 is a plasma membrane-localized soluble N-ethylmaleimide–sensitive factor attachment protein receptors (SNARE) involved in Fc receptor (FcR)-mediated phagocytosis. However, the regulatory mechanism underlying its function remains elusive. Using phosphorylation-specific antibodies, SNAP-23 was found to be phosphorylated at Ser95 in …SNAP Cell. The SNAP-tag ® system is based on a DNA repair enzyme, O 6 -alkylguanine-DNA alkyltransferase (AGT). It allows for multiple substrate options to enable color changes. It is highly temperature and fixation stable and can be used in vitro or in vivo . The substrate consists of two parts; the benzylguanine group and the functional ...Gen Mobile. Gen Mobile is a MVNO that operates on T-Mobile's network. Its Unlimited Talk, Text & 7GB Data plan $30/month (previously free through the ACP). That plan includes 7GB of high-speed data and can be used with a mobile hotspot. You also get unlimited calling to over 100 international destinations.Laboratory Methods in Enzymology: Cell, Lipid and Carbohydrate. Kirstie Canene-Adams, in Methods in Enzymology, 2013. 1 Theory. Fixation: There are four general procedures for tissue fixation; snap freezing, OCT media embedding, RNA stabilizing reagent, or fixative.This protocol will focus on formalin fixation since it is the most common.Clicking this will show the snap options. Selecting (none) means the notes or clips will slide freely, other options like ' line ' will mean the notes/clips will jump 1 line-unit left/right as you drag them, in other words, 'snap to' the lines. Check the manual for other snap modes. Generally it's best to use FL Studio with snap enabled, 'line ...SNAP-Cell ® Oregon Green ® is a photostable green fluorescent substrate that can be used to label SNAP-tag ® fusion proteins inside living cells or in vitro. Conjugates of Oregon …When it comes to choosing the perfect clothing for seniors, comfort and ease of use are paramount. Snap front robes are an excellent option as they provide convenience and simplici...The vivo X90 Pro Plus is a successor to the excellent vivo X80 Pro and X70 Pro Plus. It embodies the spirit of the Snapdragon 8 Gen 2 in its entirety, with a slew of features that are overkill for ...The process of applying for free cell phone service is thankfully pretty easy—you can just go to the website of a cell phone company that participates in the program. For example, if you wanted to check out Assurance Wireless, there’s a big “Apply Now” button front and center. Just follow the instructions and you should find out if you ...The practice of cell biology without the use of fluorescent proteins is unimaginable today, and the purification of recombinant proteins using affinity tags is standard practice. Imaging with SNAP-tag Technology: Clone gene of interest into NEB expression vector. 2) Transfect plasmid fusion into cells, protein is expressed in cells.SNAP-Cell ® 430 is a blue fluorescent substrate that can be used to label SNAP-tag ® fusion proteins inside living cells, on cell surfaces, or in vitro. It is a cell-permeable substrate (BG-430) based on diethylaminocoumarin. It is suitable for appropriate blue lasers and filter sets. It has an excitation maximum at 421 nm and emission maxima ...SnapCell named in top 500 of Inc. 5000 as one of America’s fastest-growing private companies. Aug 17, 2022. Award-winning video creation tool SnapCell has been recognized in the Inc. 5000 Annual List, ranking at number 366 as one of the fastest-growing private companies in America. Inc. magazine recently revealed this year’s prestigious ...Apr 7, 2022 · Michael: The millenial. With recent statistics showing that millennials have become the largest car buying demographic in America, it makes sense that our first buyer persona comes from this generation. Name: Michael (the most popular name for millennials, so calling him Michael makes sense). Gender: Male. Age: 32. Atlas DX. Atlas DX is a cloud-based platform that powers Affinitiv's sales, service, and retention marketing solutions for the automotive industry. This single, fully integrated platform is built on predictive intelligence to promote initial purchase, repurchase, and long-term loyalty, accelerating performance for OEMs and dealers.Snapcell. Tampa, Florida, United States. website. Snapcell is a SaaS automotive video marketing platform that helps car dealerships build trust and improve customer engagement through personalized video solutions. Snapcell was founded in 2016 and is based in Tampa, Florida. Deal Type. Try For Free 7-Day Free Trial.07 Jul, 2020, 08:36 ET. TAMPA, Fla., July 7, 2020 /PRNewswire/ -- Automotive video tool, SnapCell has launched its brand new Snap360 feature, making it the complete digital and remote selling tool ...Welcome to SnapCell. SnapCell (Aquired by TradePending) 3.3K views1 year ago. SnapCell MPIexpress. SnapCell (Aquired by TradePending) 117 views1 year ago. SnapCell Live …May 16, 2020 · Email is still a powerful and lucrative form of marketing, providing a direct route into your customer’s inbox. Combine video with your emails, using a tool like SnapCell, and reap up to 300% open rates. 63% of emails are opened on mobile, reaching customers on the go. It is cheap, personalisable and provides a fruitful ROI of $42 dollars per ... The vivo X90 Pro Plus is a successor to the excellent vivo X80 Pro and X70 Pro Plus. It embodies the spirit of the Snapdragon 8 Gen 2 in its entirety, with a slew of features that are overkill for ...Through SnapCell, you can create detailed, high-quality video content in minutes. From a stunning 360 walkaround of a specific vehicle to a step-by-step guide on what to expect at handover, you can add SnapCell videos to your website or social media channels with ease. But you also need to make a customer feel welcome in the showroom too.Here’s an old Southern tradition for a quick and easy Christmas tree that’s perfect for the front porch, a nook in your kitchen, or even for your entryway. And all you need are tom...Super-premium domain values generally range from $10,000 to $2 million. The average industry sales price is approximately $30,000. Our marketplace also has thousands of “close out” domains, at even lower prices! Building a fabulous online presence starts with a top-quality domain name from DomainMarket.com.The vivo X90 Pro Plus is a successor to the excellent vivo X80 Pro and X70 Pro Plus. It embodies the spirit of the Snapdragon 8 Gen 2 in its entirety, with a slew of features that are overkill for ...Antikörper-freie Zellfärbung mit der SNAP-tag Technologie. Die SNAP-tag Technologie für Antikörper-freies Live Cell Imaging und viele weitere Anwendungsgebiete ist nicht zuletzt durch den Nobelpreis für Chemie 2014 für Stefan Hell in den Fokus der Forscher gerückt.Cell lines in which Nup96 is endogenously tagged with mEGFP, SNAP-tag, HaloTag or mMaple serve as versatile reference samples, enabling 3D resolution calibration, assessment of labeling efficiency ...A dealer 20 group is a small network of 12 to 20 automotive dealers with similar size operations and business models. Dealers within the same group don’t operate in the same market areas, so group members can be considered allies rather than competitors. Groups tend to meet three times a year to share ideas, best practices, successes ...Snap Objects to Grid. When trying to resize or position objects such as charts, autoshapes, or controls on a spreadsheet, with the intent of aligning them to edges of cells, you can hold down the ALT key while doing so. This will have the effect of “snapping” the object to the cells. In this image the grouped object is sized perfectly to a ...Simple, reliable, and compatible with all modern Android, iOS and Desktop devices. No more long, meandering videos as you figure out what to say. Snapcell video only records when you hold your finger down, and stops when you lift your finger. Everything is then seamlessly stitched together for an engaging customer experience.You can apply the $30 ACP discount and receive free mobile service if you choose the Unlimited Talk, Text and 7GB LTE Data Plan for Phones or the 7GB LTE Data Plan for Devices — or, when available, you may apply the $30 ACP discount to a different phone or connected device plan and pay the difference between the discount and the plan cost if the plan you choose is more …SNAP-Cell ® Block (bromothenylpteridine, BTP) is a non-fluorescent compound that blocks the reactivity of the SNAP-tag ® in solution or in living cells. SNAP-Cell Block is highly membrane permeable. Once it reacts in the cell with the SNAP-tag, it irreversibly inactivates it for subsequent labeling steps. It can be used to generate inactive ... Schedule a demo of our new or used car dealership software including vehicle walkaround videos, live video chat, live messaging, & API integrations. Thanks to each and every one of you for your loyalty and support throughout our journey together and for your trust in the SnapCell vision. SnapCell recently awarded the 2023 @Kain Automotive’s ...... SNAP-25 on serine 187, promotes NMDAR delivery to the cell surface via SNARE-dependent exocytosis (Lau et al., 2010). In the latter study the authors ...Only a change in the way that Apple protects consumers or a massive shift by consumers away from Apple could provide them with what they need....MSFT Snap, Crackle and Pop! Senate ...The SNAP-tag ® system is based on a DNA repair enzyme, O 6-alkylguanine-DNA alkyltransferase (AGT).It allows for multiple substrate options to enable color changes. It is highly temperature and fixation stable and can be used in vitro or in vivo.The substrate consists of two parts; the benzylguanine group and the functional group, which can be a …SNAP-Cell ® TMR-Star is a red fluorescent substrate that can be used to label SNAP-tag ® fusion proteins inside living cells, on cell surfaces, or in vitro. It is a cell-permeable substrate (CP-6-TMR) is based on the single isomer 6-carboxytetramethylrhodamine. It has an excitation maximum at 554 nm and an emission maximum at 580 nm.SNAP-Cell ® 505-Star is a photostable green fluorescent substrate that can be used to label SNAP-tag ® fusion proteins inside living cells, on cell surfaces, or in vitro. It is a cell-permeable substrate (CP-6-505) based on the single isomer 6-carboxyrhodamine 110. It has an excitation maximum at 504 nm and an emission maximum at 532 nm.SNAP Cell. The SNAP-tag ® system is based on a DNA repair enzyme, O 6 -alkylguanine-DNA alkyltransferase (AGT). It allows for multiple substrate options to enable color changes. It is highly temperature and fixation stable and can be used in vitro or in vivo . The substrate consists of two parts; the benzylguanine group and the functional ...010659: Cannot obtain cell size from snap raster environment.Are you looking for the latest free ringtones to customize your cell phone? Look no further. With a few simple steps, you can get the newest and hottest ringtones for your device. ...SNAP-Cell ® 647-SiR is a far-red fluorescent substrate that can be used to label SNAP-tag ® fusion proteins inside living cells, on cell surfaces, or in vitro. It is a cell-permeable substrate (also termed SiR-SNAP) based on 6-carboxy-tetramethylsiliconrhodamine. It has an excitation maximum at 645 nm and an emission maximum at 661 nm. Choose from our huge range of tools and create your custom dealership package. Get your custom quote in just a few clicks and discover how Snapcell’s solutions can help drive your dealership to the next level. Aug 15, 2023 · Aug 15, 2023. Big news today! We’re thrilled to announce that Snapcell has been acquired by TradePending. A huge thanks to all of our friends, family, co-workers, and customers for supporting us these past eight years. This is a HUGE milestone for us and we’re excited for this next chapter in our story. Read the official release below. SnapCell, now a part of TradePending: Interactive video solutions enabling you to easily, record, send, measure and humanize your messages to build rapport with your customers making more sales ...SNAP-tag fusion proteins labeled with SNAP-Cell TMR-Star should have an excitation maximum at 554 nm and an emission maximum at 580 nm, and can be imaged with standard rhodamine filter sets. We recommend routinely labeling one well of non-transfected or mock-transfected cells as a negative control.Welcome Snapcell users to your new TradePending sign in. Email. Continue.SnapCell helps you build rapport with customers and make more sales through the power of personal video. SnapCell is a simple & reliable automotive video tool that increases: - Dealership Revenue. - Showroom Visits. - Service Upsell. - CSI Scores. Download the app today and begin to capture the attention of your customers. SNAP-Cell ® Oregon Green ® is a photostable green fluorescent substrate that can be used to label SNAP-tag ® fusion proteins inside living cells or in vitro.Conjugates of Oregon Green are more photostable than those of fluorescein, and their fluorescence properties are essentially pH insensitive in the physiological pH range. SNAP Switch enables quantitative detection of trafficking to locations of interest within live cells using flow cytometry. Using SNAP Switch, we followed the …Nov 16, 2022 · Go to www.LifeWireless.com and click "Add Minutes/Data". If you have a smart phone, go to your MyLifeWireless app and click "Add Minutes". Call Customer Service at 1-888-543-3620, or by dialing 611 on your Life Wireless phone. You can also go to any Moneygram location and use Receive Code: 7924. Clicking this will show the snap options. Selecting (none) means the notes or clips will slide freely, other options like ' line ' will mean the notes/clips will jump 1 line-unit left/right as you drag them, in other words, 'snap to' the lines. Check the manual for other snap modes. Generally it's best to use FL Studio with snap enabled, 'line ...Amazon.com: Case for Consumer Cellular Verve Snap Flip Phone (2022), Nakedcellphone [Black Vegan Leather] Form-Fit Cover with [Built-in Screen Protection] and [Metal Belt Clip] for Z2336CC ... Military Grade Cell Phone Case,Rugged Pouch Holster Nylon Metal Clip Belt Flip Phone Case Fits Kyocera Cadence S2720, DuraXTP, DuraXV LTE, DuraXV Plus ...Jan 14, 2024 ... Yet another clean-up effort is underway across Canberra after a major storm struck the capital for the third time in just over a month.Blocking agents are non-fluorescent compounds that block the reactivity of the SNAP- or CLIP-tag intracellularly (SNAP-Cell® Block, NEB #S9106; and CLIP-Cell™ Block, NEB #S9220) or on the surface of live cells (SNAP-Surface® Block, NEB #S9143; and CLIP-Cell Block).They can be used to generate inactive controls in live cell labeling experiments performed with SNAP- or …Welcome Snapcell users to your new TradePending sign in. Email. Continue.Cell. 2006 Dec 15;127(6):1286-7. doi: 10.1016/j.cell.2006.12.005. Authors Matthew S Hayden 1 , A Phillip West, Sankar Ghosh. Affiliation 1 Departments of Immunobiology and Molecular Biophysics and Biochemistry, Yale University School of Medicine, NewHaven, CT 06519, USA. PMID: 17174900 DOI ...Validate the SNAP-tag fusion protein expression when the cells reach 80% confluency by incubating 10 µl of the culture supernatant from the transfected HEK293T cells with 0.05 nM of SNAP-Surface ...Wall Street isn't happy with the social media darling. To stay alive in its brutish war against Facebook, one of the most important things for Snap to do is to grow the number of p...Tea snap is a term used to describe a type of tea that is brewed with high-quality tea leaves and infused with natural flavors. It is gaining popularity among tea lovers due to its...

SnapCell General Information Description. Developer of an automotive video platform designed to humanize customers' buying experience. The company's platform is an interactive video tool that offers live video chat, texting straight through the video, inventory integration and oversight and reporting, enabling clients to build rapport with their …. Streaming lifetime

snap cell

Welcome Snapcell users to your new TradePending sign in. Email. Continue. Ongoing Support. Once Snapcell is firmly established at your dealership, the hard part is over. Staff will start seeing the increased conversion and higher customer satisfaction that comes with personal video. But our job isn’t done — Leaderboards and encouraging push notifications within the Snapcell app keep your team engaged, and with ... Jul 7, 2020 · 07 Jul, 2020, 08:36 ET. TAMPA, Fla., July 7, 2020 /PRNewswire/ -- Automotive video tool, SnapCell has launched its brand new Snap360 feature, making it the complete digital and remote selling tool ... Mar 17, 2021 · Here at SnapCell, as we put together a guide to creating the perfect walk around video, we realized this is an essential part of video’s growing demand. After all, this need for better instructions is just one of the reasons ‘how to’, unboxing, and other video content based on walk arounds are so popular. But they also provide dealers ... Sep 8, 2020 ... SNAPSwitch enables quantitative detection of trafficking to locations of interest within live cells using flow cytometry. Using SNAPSwitch, we ...With the SnapCyte™ app and companion SnapCyte™ Adapter, you can use any lab microscope to measure cell growth. A New Way of Thinking Cell Growth Data. The ...Aug 15, 2023 · Adds video engagement tool to personalize car dealership interactions. Chapel Hill, NC, August 15, 2023 – TradePending, a sales enablement and consumer experience software platform for car dealerships, announced today that it has acquired Snapcell, a SaaS automotive video marketing platform that helps car dealerships build trust and improve ... 2. Brand videos. Allow potential customers to really get to know your brand, in order to build credit and trust. This could include a video of your dealership, getting to know your team or the brand values. If a potential customer then walks into the dealership, they’re more likely to spot a familiar face. 3.Snap fishing is a popular angling technique that involves quickly jerking or pulling the fishing line to mimic the movement of prey in the water, enticing fish to bite. This techni...SNAP-Cell ® Oregon Green ® is a photostable green fluorescent substrate that can be used to label SNAP-tag ® fusion proteins inside living cells or in vitro.Conjugates of Oregon Green are more photostable than those of fluorescein, and their fluorescence properties are essentially pH insensitive in the physiological pH range.For over 30 years, SNAP has deducted the cost of telephones in determining a household's eligibility and benefit amounts, either by deducting actual telephone bills or standard telephone allowances. ... What is common in all these situations is that there is a specific device, even in the case of disposable cell phones. Also in this regard ...Are you looking for a reliable cell phone retailer? With so many options available, it can be hard to know which one is right for you. To help you out, we’ve put together a guide t....

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